Controlled drug delivery from metronidazole-containing bioactive endodontic cements.

OBJECTIVES: This study aims to formulate metronidazole liquid nanocapsules (MTZLNC) and evaluate their effect on the physicochemical and biological properties of calcium silicate-based bioactive endodontic cements, in vitro. METHODS: A MTZLNC suspension was formulated by deposition of the preformed polymer and characterized by laser diffraction and high-performance liquid chromatography (HPLC). Calcium silicate (CS) was mixed with a radiopaque agent (calcium tungstate - CaWO4), at 10 wt%, to produce the cement powder. Cements liquids were used with two concentrations of MTZLNC suspension: 0.3 mg/ml and 0.15 mg/ml. Cements prepared with distilled water were used as the control. The radiopacity, setting time, and flow were evaluated following ISO 6876:2012. The compressive strength analysis was conducted according to ISO 9917:2007. pH and mineral deposition were evaluated after immersion in simulated body fluid (SBF). Cell behavior was evaluated by the viability of pre-osteoblastic cells and pulp fibroblasts by SRB and MTT and the antibacterial activity against Enterococcus faecalis was analyzed immediately and after nine months of water storage. RESULTS: MTZLNCs were formulated with a median diameter of 148 nm and 83.44 % load efficiency. Increased flow and reduced strength were observed for both MTZLNCs concentrations. The incorporation of MTZLNCs maintained the ability of cements to increase pH media and promote mineral deposition over the samples, without promoting cytotoxicity. A 2 log10 reduction in E. faecalis CFU was observed immediately and after nine months in water storage. CONCLUSION: The formulation of MTZLNCs allowed the development of antibacterial calcium silicate-based-cements with suitable physicochemical properties and bioactivity, with a reduction in mechanical strength. The 0.3 mg/ml concentration in cements liquid promoted effective and sustainable antibacterial activity.

Niobium-containing bioactive glasses modulate alkaline phosphatase activity during bone repair.

This study aimed to evaluate the pre-clinical behavior of niobium-containing bioactive glasses (BAGNb) by their ability to promote bone repair and regulate alkaline phosphatase (ALP) levels in an animal model. BAGNbs were produced as powders and as scaffolds and surgically implanted in the femur of male rats (Wistar lineage n = 10). Glasses without Nb (BAG) were produced and implanted as well. The Autogenous Bone (AB) was used as a control. After 15, 30, and 60 days of surgical implantation, blood serum samples were collected to quantify ALP activity, and femurs were removed to assess bone repair. Bone samples were histologically processed and stained with H&E to quantify the % new bone into defects. No postoperative complications were identified. Early-stage repair (15 days) resulted in increased ALP activity for all groups, with increased values ​​for powdered BAGNb. The maturation of the new bone led to a reduction in serum ALP levels. Histological sections showed the formation of immature bone tissue and vascularization with the progression of bone deposition to mature and functional tissue over time. BAG powder showed less new bone formation in 15 days, while the analysis at 30 and 60 days showed no difference between groups (p > .05). Niobium-containing bioactive glasses safely and successfully induced bone repair in vivo. The modulation of ALP activity may be a pathway to describe the ability of niobium-containing materials to contribute to new bone formation.

Synthesis of silver core-shell nanoparticles and their influence on an experimental resin endodontic sealer: An in vitro analysis.

AIM: To avoid root canal recontamination and endodontic treatment failure, endodontic sealers with antibacterial activity could be an alternative. Silver nanoparticles have antibacterial activity and this study aimed to synthesize Ag@SiO2 nanoparticles, incorporate them into an experimental endodontic resin sealer and evaluate their influence on physicochemical and biological properties. METHODOLOGY: Ag@SiO2 nanoparticles were produced using the sol-gel process, based on the Stöber method. The particles were characterized in terms of their chemical structure by Fourier transform-infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA), UV-Vis spectral analysis, scanning electron microscopy, and transmission electron microscopy, where the particle morphology and diameter were analysed. A dual-cured experimental endodontic resin sealer was formulated using 70 wt% UDMA, 15 wt% GDMA, and 15 wt% BisEMA. The photoinitiators were added separately in two pastes. The Ag@SiO2 nanoparticles were incorporated into the endodontic sealer at the concentrations of 2.5 wt%, 5 wt%, and 10 wt%, and a control group without nanoparticles was also formulated. The endodontic sealers were evaluated for their flow, film thickness, degree of conversion, softening in solvent, radiopacity, cytotoxicity and antibacterial activity immediately and after 9 months in water storage. RESULTS: Silver was detected in the chemical characterization of Ag@SiO2 that presented a spheric regular shape and average 683.51 nm ± 93.58 diameter. Sealers presented adequate flow and film thickness while radiopacity values were below the ones required by ISO 6876. All groups underwent softening after immersion in a solvent. The 10 wt% groups showed a higher loss of subsurface hardness (∆KHN%). No reduction in cell viability was observed. Enterococcus faecalis viability in biofilm was reduced in 10 wt% groups after 24 h and 9 months. CONCLUSION: The addition of 10 wt% Ag@SiO2 reduced E. faecalis viability at immediate and longitudinal analysis while maintaining the physicochemical properties of developed sealers.

Orthodontic resins loaded with niobium silicate particles: Impact of filler concentration on the physicochemical and biological properties.

OBJECTIVES: White spot lesions (WSL) are prevalent in patients using orthodontic appliances. The presence of ion-releasing compounds in the tooth-appliance interface may limit enamel demineralization to control WSL incidence. Thus, this study aims to evaluate the mineral formation on SiNb-containing experimental orthodontic resins and the influence of these fillers on the physicochemical and biological properties of developed materials. MATERIALS AND METHODS: The SiNb particles were synthesized via the sol-gel route and characterized by their molecular structure and morphology. Photopolymerizable orthodontic resins were produced with a 75 wt% Bis-GMA/25 wt% TEGDMA and 10 wt%, 20 wt%, or 30 wt% addition of SiNb. A control group was formulated without SiNb. These resins were tested for their degree of conversion, softening in solvent, cytotoxicity in fibroblasts, flexural strength, shear bond strength (SBS), and mineral deposition. RESULTS: The addition of 10 wt% of SiNb did not impair the conversion of monomers, cytotoxicity, and flexural strength. All groups with SiNb addition presented similar softening in solvent. The presence of these particles did not affect the bond strength between metallic brackets and enamel, with SBS values ranging from 16.41 to 18.66 MPa. The mineral deposition was observed for all groups. CONCLUSION: The use of niobium silicate as filler particles in resins may be a strategy for the adhesion of orthodontic appliances. The 10 wt% SiNb concentration resulted in a material with suitable physicochemical and biological properties while maintaining the bond strength to tooth enamel and promoting mineral deposition.

Antibacterial Effect of Triazine in Barrier Membranes with Therapeutic Activity for Guided Bone Regeneration.

Objective: This study aimed to develop polymer-based barrier membranes based on poly(butylene-adipate-co-terephthalate) (PBAT) with the addition of 1,3,5-triacriloilhexahydro-1,3,5-triazine (TAT). Materials and Methods: Polymeric solutions were used to produce membranes with 5 wt% and 10 wt% of TAT by solvent casting. Membranes without the addition of TAT were used as controls. The membranes were chemically characterized by Fourier transform infrared spectroscopy (FTIR) and thermogravimetry (TGA); surface properties were assessed by profilometry and contact angle; the mechanical behavior was evaluated by a tensile test, and the biological properties were assessed by direct−indirect cell viability and antibacterial activity by S. mutans and S. aureus colony-forming units. Results: TAT was detected in the FTIR and TGA analyses and modified the top surface of the membranes, increasing their roughness and wetness in both concentrations compared to the control group (p < 0.05). The addition of TAT, regardless of concentration, reduced the tensile strength and increased membrane stiffness (p < 0.05). The cell viability of 5 wt% TAT and 10 wt% TAT was 86.37% and 82.36%, respectively. All tested concentrations reduced the formation of biofilm on the membranes when compared to the control. Conclusion: The addition of TAT successfully resulted in the antimicrobial ability of PBAT-based barrier membranes, while it maintained acceptable levels of cell viability in membranes with adequate handling and surface properties.

Implementation in restorative treatments in public health: a 10-year analysis of resin composite procurement in Brazil.

This study aims to assess the resin composite purchases in a public health system and understand the variables associated with its cost and with the decision-making process over a period of 10 years. Secondary data collection was performed using the Brazilian Healthcare Prices Database (BDHP) from 2010 to 2019. All conventional and bulk-fill composites were selected. Date of purchase, institution type and location, procurement modality, number of purchased items, manufacture, and unit prices were collected. A multiple linear regression model assessed the influence of price in the procurement variables, while a multinomial logistic regression compared purchase probabilities between the materials. In total, 18,138 observations were collected with 2,129,294 purchased units and a total cost of USD 43,504,260.00. Conventional composites appeared in 98.5% of the procurement process. Nanofilled conventional composites were the most purchased materials, with an increased probability of purchase over time, despite its higher prices. An increase in prices was predicted for bulk-fill materials when compared to conventional ones. The odds of purchasing this category increased by 3.14x for every price increase over the years. Sociodemographic and type of institution influences the prices and the probability of procurement in the healthcare system. Nanofilled and bulk-fill resin are increasingly included in clinical practice. These findings highlight possible modifications to the standard-of-care in restorative treatments and how the translation of knowledge may occur from the development of new materials to the clinical application considering the economic impact of these modifications.

Impact of economic factors and knowledge translation on public procurement for dental adhesive systems.

This study aimed to screen the public procurement for dental adhesives in a public health system and evaluate the variables associated with the type and cost of purchased materials. A time series was conducted with secondary data collated from the Brazilian Databank of Healthcare Prices (BDHP). All public procurements for adhesive systems registered from 2010 to 2019 were collated. The frequency of purchase was described, and a multiple linear regression model was used to assess the influence of unit price on predictor variables. Multinomial logistic regression was conducted to describe the influence of selected variables on the probability of purchase for different classes of adhesive systems. A total of 2,752 purchases were analyzed, and the total cost of these materials reached US$ 7,022,659.00 in the 10 analyzed years. The 3-step Etch & Rinse adhesives corresponded to 45.63% of the processes, with increased probability of being purchased over time. The process conducted by public universities in recent years, using the auction modality, presented lower unit prices. The year of purchase, the type of institutions, the institution's location, and the procurement modality influenced the procurement process. The performed analyses contribute to the understanding of the variables associated with the procurements for adhesive systems, providing valuable information for rationalizing public expenditure and for implementing evidence-based practices to guarantee cost-effective and clinically effective procedures for users of the Brazilian public healthcare system.

Physicochemical and biological properties of experimental dental adhesives doped with a guanidine-based polymer: an in vitro study.

OBJECTIVES: The objective of this study is to formulate experimental dental adhesives with different polyhexamethylene guanidine hydrochloride concentrations (PHMGH) and evaluate their physical, chemical, and biological properties. MATERIALS AND METHODS: The experimental adhesives were formulated with 0 (control, GCTRL), 0.5 (G0.5%), 1 (G1%), or 2 (G2%) wt.% into the adhesive. The adhesives were analyzed for degree of conversion (DC%), softening in solvent (ΔKHN%), ultimate tensile strength (UTS), microtensile bond strength (µTBS) immediately and after 1 year of aging, antibacterial activity, and cytotoxicity. RESULTS: There were no differences among groups for DC%, ΔKHN%, and UTS (p > 0.05%). There were no differences between each PHMGH-doped adhesive compared to GCTRL in the immediate µ-TBS (p > 0.05). Adhesives with at least 1 wt.% of PHMGH presented better stability of µ-TBS. PHMGH-doped adhesives showed improved longitudinal µ-TBS compared to GCTRL (p < 0.05). Lower Streptococcus mutans biofilm formation was observed for PHMGH-doped adhesives (p < 0.05). There was lower viability of planktonic S. mutans in the media in contact with the samples when at least 1 wt.% of PHGMGH was incorporated (p < 0.05). The formulated adhesives showed no cytotoxicity against pulp cells (p > 0.05). CONCLUSIONS: The adhesive with 2 wt.% of PHMGH showed the highest antibacterial activity, without affecting the physicochemical properties and cytotoxicity, besides conferring stability for the dental adhesion. CLINICAL RELEVANCE: PHMGH, a positively charged polymer, conveyed antibacterial activity to dental adhesives. Furthermore, it did not negatively affect the essential physicochemical and biocompatibility properties of the adhesives. More importantly, the incorporation of PHMGH provided stability for the µ-TBS compared to the control group without this additive.

Desenvolvimento e avaliação do uso de um objeto virtual de aprendizagem com simulação virtual sobre alginato / Development and evaluation of the use of a virtual learning object with virtual simulation on alginate

O objetivo do presente estudo foi desenvolver um Objeto Virtual de Aprendizagem (OVA) sobre materiais de moldagem, com ênfase no alginato, e avaliar a influência do seu uso sobre o aprendizado teórico e habilidade clínica de estudantes de Odontologia, assim como sobre as propriedades física e mecânica material. Sessenta e quatro estudantes receberam aula teórica expositiva sobre alginato e posteriormente foram divididos em 2 grupos, Controle (n=30) e OVA (n=34). O grupo OVA teve acesso à ferramenta educacional composta de conteúdo teórico e simulação de espatulação do alginato, sendo sua habilidade avaliada. Todos os participantesrealizaram um pré-teste após a aula teórica, assim como testes de habilidade de manipulação do material,o qualfoi submetido a ensaio de resistência à compressão e reprodução de detalhes. Uma avaliação pós-teste foi realizada após quinze dias. Os resultados dos grupos foram comparados pelo teste t, modelo de regressão logística e as variáveis significativas foram incluídas no modelo de regressão múltipla. Todas as análises foram conduzidas com significância de 5%. Na simulação do OVA 75% dos usuários obtiveram nota máxima e os resultados das avaliações teóricas não indicaram diferença estatística entre os grupos. O uso do OVA está relacionado à maior habilidade clínica, com 3,76 mais chances de correta incorporação do pó. No ensaio de resistência à compressão a média dos grupos foi superior ao preconizado pela norma, OVA 0,86±0,03 e Controle 1,09±0,04. A ferramenta desenvolvida exerce influência positiva sobre a habilidade clínica e propriedade mecânica do material estudado, constituindo-se como promissora estratégia de aprendizagem virtual (AU).

The aim of the present study was to develop a Virtual Learning Object (VLO) on impression materials, with emphasis on alginate, and to evaluate the influence of its use on the theoretical learning and clinical skills of dental students, as well as on the physical properties and material mechanics. Sixty-four students received a theoretical lecture on alginate and were later divided into 2 groups, Control (n=30) and VLO (n=34). The VLO group had access to the educational tool composed of theoretical content and simulation of alginate mixing, and their ability was evaluated. All participants performed a pre-test after the theoretical class, as well as tests of ability to handle the material, which was subjected to a compressive strength test and detailreproduction. A post-test evaluation was performed after fifteen days. The results of the groups were compared by the t test, logistic regression model and significant variables were included in the multiple regression model. All analyzes were conducted with a significance of 5%. In the VLO simulation, 75% of the users obtained the maximum score and the results of the theoretical assessments did not indicate a statistical difference between the groups. The use of VLO is related to greater clinical skill, with 3.76 more chances of correct incorporation of the powder. In the compressive strength test, the mean of the groups was higher than that recommended by the standard, VLO 0.86±0.03 and Control 1.09±0.04. The developed tool has a positive influence on theclinical ability and mechanical property of the material studied, constituting itself as a promising strategy of virtual learning (AU).

3D printing of poly(butylene adipate-co-terephthalate) (PBAT)/niobium containing bioactive glasses (BAGNb) scaffolds: Characterization of composites, in vitro bioactivity, and in vivo bone repair.

This study aimed to produce poly(butylene adipate-co-terephthalate) (PBAT)/niobium containing bioactive glasses (BAGNb) composites scaffolds produced by fused deposition modeling (FDM) printing and evaluate their physicochemical and biological properties in vitro and in vivo. The composite filaments were produced by melt-extrusion with the addition of 10 wt% of BAGNb (PBAT/BAGNb). Filaments without BAGNb were produced as the control group (PBAT). The filaments were characterized and were used to produce 3D-printed scaffolds using FDM. The scaffolds' structure and surface properties were assessed. In vitro cell, proliferation, and cell mineralization analysis were performed. In vivo data was obtained in the rat femur model (n = 10), and the bone repair was assessed after 15, 30, and 60 postoperative days. The printed structures presented 69.81% porosity for the PBAT/BAGNb group and 74.54% for the PBAT group. Higher cell mineralization was observed for the PBAT/BAGNb group. The in vivo data showed that the PBAT/BAGNb presented new bone formation comparable to positive controls. The combination of PBAT and BAGNb in 3D-printed scaffolds may be an alternative to produce bioactive materials with controllable shapes and properties for bone regeneration treatments.

Non-thermal plasma for surface treatment of inorganic fillers added to resin-based cements.

OBJECTIVES: This study aims to evaluate the effect of non-thermal plasma (NTP) surface treatment in two composite inorganic fillers and evaluate their impact on the chemical-mechanical properties and bond strength ability of experimental resin cements. MATERIALS AND METHODS: Ytterbium fluoride (YF) and barium silicate glass (BS) were characterized and submitted to different surface treatments: non-thermal plasma (NTP); non-thermal plasma and 3-(trimethoxysilyl) propyl methacrylate silanization; and 3-(trimethoxysilyl) propyl methacrylate silanization. Untreated fillers were used as a control. The fillers were incorporated at 65wt% concentration into light-cured experimental resin cements (50wt% BisGMA; 25wt% UDMA; 25wt% TEGDMA; 1mol% CQ). The degree of conversion, the flexural strength, and the microshear bond strength (µSBS) were evaluated to characterize developed composites. RESULTS: YF and BS were successfully cleaned with NTP treatment. Nor NTP neither the silanization affected the degree of conversion of resin cements. The NTP predicted an increase in YF-containing resin cements flexural strength, reducing the storage impact in these materials. NTP treatment did not affect the µSBS when applied to YF, while silanization was effective for BS-containing materials. CONCLUSION: NTP treatment of inorganic particles was possible and was shown to reduce the amount of organic contamination of the particle surface. YF surface treatment with NTP can be an alternative to improve the organic/inorganic interaction in resin composites to obtain materials with better mechanical properties. CLINICAL RELEVANCE: Surface cleaning with NTP may be an alternative for particle surface cleaning to enhance organic-inorganic interaction in dental composites resulting in improved mechanical strength of experimental resin cements.

Impact of economic factors and knowledge translation on public procurement for dental adhesive systems

Abstract: This study aimed to screen the public procurement for dental adhesives in a public health system and evaluate the variables associated with the type and cost of purchased materials. A time series was conducted with secondary data collated from the Brazilian Databank of Healthcare Prices (BDHP). All public procurements for adhesive systems registered from 2010 to 2019 were collated. The frequency of purchase was described, and a multiple linear regression model was used to assess the influence of unit price on predictor variables. Multinomial logistic regression was conducted to describe the influence of selected variables on the probability of purchase for different classes of adhesive systems. A total of 2,752 purchases were analyzed, and the total cost of these materials reached US$ 7,022,659.00 in the 10 analyzed years. The 3-step Etch & Rinse adhesives corresponded to 45.63% of the processes, with increased probability of being purchased over time. The process conducted by public universities in recent years, using the auction modality, presented lower unit prices. The year of purchase, the type of institutions, the institution's location, and the procurement modality influenced the procurement process. The performed analyses contribute to the understanding of the variables associated with the procurements for adhesive systems, providing valuable information for rationalizing public expenditure and for implementing evidence-based practices to guarantee cost-effective and clinically effective procedures for users of the Brazilian public healthcare system.

Physicochemical and biological evaluation of a triazine-methacrylate monomer into a dental resin.

OBJECTIVES: This study aimed to (1) formulate blend resins with 2.5 or 5 wt.% of the methacrylate monomer 1,3,5-triacryloylhexahydro-1,3,5-triazine (TAT), and (2) to evaluate the blend resins regarding the physicochemical and biological properties. METHODS: The base resin was formulated mixing 60 wt.% of bisphenol A glycol dimethacrylate and 40 wt.% of triethylene glycol dimethacrylate with photoinitiator/co/initiator system. TAT was added at 2.5 (G2.5%) or 5 (G5%) wt.%, and a group without TAT was used as control (Gctrl). The resins were analyzed for degree of conversion (DC), Knoop hardness (KHN), softening in solvent (ΔKHN), ultimate tensile strength (UTS), contact angle, surface free energy (SFE), antibacterial activity against Streptococcus mutans biofilm formation, and cytotoxicity against human keratinocytes. RESULTS: There was no difference for the DC (p = 0.676). The addition of TAT at 5 wt.% induced higher KHN (p<0.001), higher resistance against softening in solvent (p<0.001), and higher UTS (p = 0.04). There were no statistically significant differences for contact angle with water (p = 0.106), α-bromonaphtalene (p = 0.454), and SFE (p = 0.172). The higher the TAT concentration, the higher the antibacterial activity (p<0.001). G2.5% showed no cytotoxicity compared to Gctrl (p>0.05), and G5% induced lower cell viability (p<0.05). CONCLUSIONS: The addition of 2.5 wt.% of TAT is suitable for conveying antibacterial activity for dental resins without changing the physicochemical properties or impairing the cytotoxic effect. CLINICAL RELEVANCE: Methacrylate monomers that decrease bacterial viability and copolymerize with the resin matrix are exciting approaches to developing therapeutic materials. TAT showed promising properties to may hamper and prevent carious lesions when incorporated into dental materials. Further evaluations with higher cariogenic challenges will be carried to analyze the formulated materials.

The influence of the particle's size on the setting reaction of sol-gel derived calcium silicate particles / A influência do tamanho de partícula na reação de presa de cimentos de silicate de cálcio produzidos por sol-gel

Introduction: This study aims to analyze the influence of particles size of sol-gel derived calcium silicate particles in the setting reaction of bioactive endodontic cements. Materials and Methods: Sol-gel derived calcium silicate particles were synthesized and sieved to separate the particles in different sizes: CS400, CS200, and CS100. A commercial MTA (Control) was used as control. The particle size and the specific surface area were assessed by laser diffraction and nitrogen adsorption. The cements were prepared with water as the liquid for the reaction. The setting time was conducted according to ISO 6876, and the setting kinetics was analyzed by Fourier trans-formed infrared spectroscopy (FTIR) at different time points between 120s to 72h. Results: The particle size varied from 9.45µm (CS400 ) to 31.01 (Control). The higher specific surface area valuer reached 15.14g/cm2 in the CS400. The smallest particle sizes, the higher specific surface area, and the lowest setting time were found for CS400 (p < 0.05). Control presented the highest setting time (p < 0.05). The FTIR analyses showed the differences in materials structure over time, with faster hydration and crystallization for CS400. The setting kinetics was slower for Control even when compared to a sol-gel derived group with similar particle size. Conclusion:The route of synthesis and the particle size influences the setting reaction of calcium silicate-based cements. The reduction of particle size for sol-gel derived calcium silicates lead to the acceleration of the setting reaction of the produced bioactive endodontic cement.

Introdução: Este estudo tem como objetivo analisar a influência do tamanho de partículas de silicato de cálcio derivadas de sol-gel na reação de presa de cimentos endodônticos bioativos. Materiais e Métodos: Partículas de silicato de cálcio derivadas de sol-gel foram sintetizadas e peneiradas para separar as partículas em diferentes tamanhos: CS400, CS200 e CS100. Um MTA comercial (Controle) foi usado como controle. O tamanho das partículas e a área superficial específica foram avaliados por difração a laser e adsorção de nitrogênio. Os cimentos foram preparados com água como líquido para a reação. O tempo de presa foi conduzido de acordo com a ISO 6876, e a cinética de presa foi analisada por espectroscopia de infravermelho transformada de Fourier (FTIR) em diferentes pontos de tempo entre 120s a 72h. Resultados: O tamanho de partícula variou entre 9,45µm (CS400) e 31,01 (Controle). A maior área de superfície foi encontrada nas partículas do grupo CS400 (15.14g/cm2). Os menores tamanhos de partícula, a maior área de superfície específica e o menor tempo de presa foram encontrados para CS400 (p < 0,05). O Control apresentou o maior tempo de presa (p < 0,05). As análises de FTIR mostraram as diferenças na estrutura dos materiais ao longo do tempo, com hidratação e cristalização mais rápidas para CS400. A cinética de presa foi mais lenta para Control mesmo quando comparado a um grupo derivado de sol-gel com tamanho de partícula semelhante. Conclusão: A rota de síntese e o tamanho das partículas influenciam a reação de endurecimento dos cimentos à base de silicato de cálcio. A redução do tamanho de partícula para silicatos de cálcio derivados de sol-gel leva à aceleração da reação de pega do cimento endodôntico bioativo produzido.

Polybutylene-adipate-terephthalate and niobium-containing bioactive glasses composites: Development of barrier membranes with adjusted properties for guided bone regeneration.

This study aimed to develop bioactive guided bone regeneration (GBR) membranes by manufacturing PBAT/BAGNb composites as casting films. Composites were produced by melt-extrusion, and BAGNb was added at 10 wt%, 20 wt%, and 30 wt% concentration. Pure PBAT membranes were used as a control (0wt%BAGNb). FTIR and thermogravimetric analysis characterized the composites. Barrier membranes were produced by solvent casting, and their mechanical and surface properties were assessed by tensile strength test and contact angle analysis, respectively. The ion release and cell behavior were evaluated by pH, cell proliferation, and mineralization. Composites were successfully produced, and the chemical structure showed no interference of BAGNb in the PBAT structure. The addition of BAGNb increased the stiffness of the membranes and reduced the contact angle, increasing the roughness in one side of the membrane. Sustained pH increment was observed for BAGNb-containing membranes with increased proliferation and mineralization as the concentration of BAGNb increases. The incorporation of up to 30 wt% of BAGNb into PBAT barrier membranes was able to maintain adequate chemical-mechanical properties leading to the production of materials with tailored surface properties and bioactivity. Finally, this biomaterial class showed outstanding potential and may contribute to bone formation in GBR procedures.

The golden proportion concept: smile makeover with an 18-month follow-up.

AIM: The altered size, shape or position of the teeth affect their proper alignment, the harmony of the smile, and the dentofacial composition. The aim of this article is to describe a minimally invasive approach to improve the esthetics of a patient through the golden proportion (GP) concept with the use of direct composite resin stratification. MATERIALS AND METHODS: A 26-year-old female complained about the appearance of her smile due to the existing spaces between her anterior teeth; her small, peg-shaped lateral incisors; the wear of her canines; and the color of her teeth. The clinical examination confirmed diastemas in the anterior teeth, peg-shaped maxillary lateral incisors, and incisal wear of the canines. Treatment with dental whitening followed by composite restorations was performed in line with the current conservative approach in dentistry. The patient was followed up for 18 months. CONCLUSION: The use of the additive technique with composite for the closure of diastemas under the GP concept illustrates an example of viable management to restore esthetic harmony through a minimally invasive approach, with reliability over time and the advantages of being less laborious, less time consuming, and less expensive than other approaches. The treatment plan was acceptable to the patient.

Ionic liquid-loaded microcapsules doped into dental resin infiltrants.

Resin infiltrants have been effectively applied in dentistry to manage non-cavitated carious lesions in proximal dental surfaces. However, the common formulations are composed of inert methacrylate monomers. In this study, we developed a novel resin infiltrant with microcapsules loaded with an ionic liquid (MC-IL), and analyzed the physical properties and cytotoxicity of the dental resin. First, the ionic liquid 1-n-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide (BMI.NTf2) was synthesized. BMI.NTf2 has previously shown antibacterial activity in a dental resin. Then, MC-IL were synthesized by the deposition of a preformed polymer. The MC-IL were analyzed for particle size and de-agglomeration effect via laser diffraction analysis and shape via scanning electron microscopy (SEM). The infiltrants were formulated, and the MC-IL were incorporated at 2.5%, 5%, and 10 wt%. A group without MC-IL was used as a control. The infiltrants were evaluated for ultimate tensile strength (UTS), contact angle, surface free energy (SFE), and cytotoxicity. The MC-IL showed a mean particle size of 1.64 (±0.08) µm, shriveled aspect, and a de-agglomeration profile suggestive of nanoparticles' presence in the synthesized powder. There were no differences in UTS among groups (p > 0.05). The incorporation of 10 wt% of MC-IL increased the contact angle (p < 0.05), while the addition from 5 wt% reduced the SFE in comparison to the control group (p < 0.05). The human cell viability was above 90% for all groups (p > 0.05). The incorporation of microcapsules as a drug-delivery system for ionic liquids may be a promising strategy to improve dental restorative materials.

Incorporation of amoxicillin-loaded microspheres in mineral trioxide aggregate cement: an in vitro study.

OBJECTIVES: In this study, we investigated the potential of amoxicillin-loaded polymeric microspheres to be delivered to tooth root infection sites via a bioactive reparative cement. MATERIALS AND METHODS: Amoxicillin-loaded microspheres were synthesized by a spray-dray method and incorporated at 2.5% and 5% into a mineral trioxide aggregate cement clinically used to induce a mineralized barrier at the root tip of young permanent teeth with incomplete root development and necrotic pulp. The formulations were modified in liquid:powder ratios and in composition by the microspheres. The optimized formulations were evaluated in vitro for physical and mechanical eligibility. The morphology of microspheres was observed under scanning electron microscopy. RESULTS: The optimized cement formulation containing microspheres at 5% exhibited a delayed-release response and maintained its fundamental functional properties. When mixed with amoxicillin-loaded microspheres, the setting times of both test materials significantly increased. The diametral tensile strength of cement containing microspheres at 5% was similar to control. However, phytic acid had no effect on this outcome (p > 0.05). When mixed with modified liquid:powder ratio, the setting time was significantly longer than that original liquid:powder ratio (p < 0.05). CONCLUSIONS: Lack of optimal concentrations of antibiotics at anatomical sites of the dental tissues is a hallmark of recurrent endodontic infections. Therefore, targeting the controlled release of broad-spectrum antibiotics may improve the therapeutic outcomes of current treatments. Overall, these results indicate that the carry of amoxicillin by microspheres could provide an alternative strategy for the local delivery of antibiotics for the management of tooth infections.

Avaliação de soluções antifúngicas de guanidina para resina base de dentadura: um estudo in vitro / Evaluation of guanidine antifungal solutions for denture base resin: an in vitro study

Background: The purposes of this study were: (1) to formulate polyhexamethylene guanidine hydrochloride (PHMGH) solutions at different concentrations; (2) to evaluate their antifungal activity against a mature biofilm of Candida albicans on acrylic resins; (3) to evaluate possible effects on acrylic resins flexural strength and surface roughness. Methods: PHMGH solutions were formulated with distilled water and 0.125, 0.250, or 0.5 wt.% of PHMGH. One group without PHMGH was used as control. For antifungal activity analysis, acrylic resin specimens were contaminated with C. albicans. Specimens were immersed in PHMGH solutions or distilled water for 5 or 10 min. Ultimate flexural strength and surface roughness of acrylic resins were evaluated. Results: All PHMGH solutions at 5 or 10 min showed antifungal activity compared to the control group (p<0.05). The group with 0.5 wt.% of PHMGH showed no countable colonies regardless of the time of contact with the acrylic resins. After 10 min, all PHMGH solutions had antifungal effect without differences from 0.125 to 0.5 wt.% of PHMGH. All groups showed high flexural strength after contact with the solutions compatible with ISO 20795-1:2013 recommendation. The values of surface roughness always remained low, from 0.01 to 0.04 µm for all groups. Conclusion: Within the limitations of this study, it was concluded that the use of a solution composed by distilled water and 0.5 wt.% of PHMGH for 5 min was effective as a disinfectant agent against mature biofilm of C. albicans , maintaining acceptable roughness and flexural strength.

Antecedentes: Os objetivos deste estudo foram: (1) formular soluções de cloridrato de polihexametileno guanidina (PHM-GH) em diferentes concentrações; (2) avaliar sua atividade antifúngica contra biofilme maduro de Candida albicans em resinas acrílicas; (3) avaliar efeitos na resistência à flexão e rugosidade da superfície. Métodos: Soluções de PHMGH foram formuladas com água destilada e 0,125, 0,250 ou 0,5% em peso de PHMGH. Um grupo sem PHMGH foi usado como controle. Para análise da atividade antifúngica, amostras de resina acrílica foram contaminadas com C. albicans, e imersas em soluções de PHMGH ou água destilada por 5 ou 10 min. Resistência à flexão final e rugosidade da superfície foram avaliadas. Resultados: Todas as soluções de PHMGH aos 5 ou 10 minutos apresentaram atividade antifúngica em comparação ao grupo controle (p <0,05). O grupo com 0,5% em peso de PHMGH não mostrou colônias contáveis, independentemente do tempo. Após 10 minutos, todas as soluções de PHMGH tiveram efeito antifúngico sem diferenças de 0,125 a 0,5% em peso de PHM-GH. Os grupos apresentaram alta resistência à flexão após o contato com as soluções compatíveis com a recomendação ISO 20795-1: 2013. Os valores de rugosidade da superfície permaneceram baixos, de 0,01 a 0,04 µm para todos os grupos. Conclusão: Dentro das limitações deste estudo, concluiu-se que o uso de uma solução composta por água destilada e 0,5% em massa de PHMGH por 5 min foi eficaz como agente desinfetante contra o biofilme maduro de C. albicans, mantendo rugosidade e resistência à flexão aceitáveis.

Niobium silicate particles as bioactive fillers for composite resins.

OBJECTIVES: This study aimed to evaluate the influence of niobium silicate (SiNb) particles in the physicomechanical and biological properties of an experimental composite resin. METHODS: The SiNb particles were incorporated (50 wt%) into a polymeric matrix formulated with 70 wt% Bisphenol A-Glycidyl Methacrylate and 30 wt% Triethylene Glycol Dimethacrylate to formulate an experimental composite resin. A control group was formulated with barium silicate glass (SiBa) as filler for the same polymeric matrix. The composite resins were tested for their refractive index, polymerization kinetics, flexural strength, radiopacity, softening in solvent, pH, cytotoxicity and mineral deposition. RESULTS: The SiBa group presented refractive index results between 1.50 and 1.52 and the SiNb between 1.43-1.45. No statistically significant difference in the degree of conversion, flexural strength, and softening in solvent was observed between different groups. Radiopacity was lower for SiNb, while the addition of these particles increased cell viability. The pH was increased for all groups after immersion. The mineral deposition analysis resulted in increased deposition above specimens after the immersion in SBF. SIGNIFICANCE: Niobium silicate particles may be used as an alternative inorganic filler to achieve an adequate balance between physical-chemical and biological properties for the development of bioactive composite resins.